De novo transcriptome assembly and functional annotation of the southern rock lobster (Jasus edwardsii)

Souza, Carla Anjos, Murphy, Nicholas, and Strugnell, Jan M. (2018) De novo transcriptome assembly and functional annotation of the southern rock lobster (Jasus edwardsii). Marine Genomics, 42. pp. 58-62.

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Southern rock lobsters (Jasus edwardsii) are a valuable commodity and comprise important fishery resources in Australia and New Zealand. Population genomic methods (e.g. reduced representation sequencing) have been used to investigate patterns of dispersal and recruitment and the role of local adaptation within rock lobster species. However, application of these methods is constrained by the lack of pre-existing reference genome or transcriptome for Jasus and/or closely related species. In this study, we build a reference transcriptome assembly of 187,981 transcripts for Jasus edwardsii based on RNA sequencing data of four tissues (eyestalk, green gland, hepatopancreas and supraesophageal ganglion). This work will provide a significant resource to aid in investigation of the molecular basis of biological and evolutionary processes relevant to the fishing industry of spiny lobster and related taxonomic groups.

Item ID: 56902
Item Type: Article (Research - C1)
ISSN: 1876-7478
Keywords: Transcriptome assembly, Jasus edwardsii, RNA-seq, spiny lobsters
Copyright Information: © 2018 Elsevier B.V. All rights reserved
Funders: Australian Research Council (ARC), Brazilian Program Science without Borders (BPSWB)
Projects and Grants: ARC Project No. DP150101491, BPSWB Postdoctoral research grant
Date Deposited: 16 Jan 2019 07:46
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310902 Animal cell and molecular biology @ 100%
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