Influenza-specific antibody-dependent phagocytosis

Ana-Sosa-Batiz, Fernanda, Vanderven, Hillary, Jegaskanda, Sinthujan, Johnston, Angus, Rockman, Steven, Laurie, Karen, Barr, Ian, Reading, Patrick, Lichtfuss, Marit, and Kent, Stephen J. (2016) Influenza-specific antibody-dependent phagocytosis. PLoS ONE, 11 (4). e0154461.

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Background Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.

Methods We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.

Results We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity in vitro.

Conclusion We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection in vitro. Repeated exposure of humans to multiple IAV infections likely leads to the development of ADP that is cross-reactive to strains not previously encountered. Further analyses of the protective capacity of broadly reactive influenza-specific ADP is warranted.

Item ID: 56886
Item Type: Article (Research - C1)
ISSN: 1932-6203
Copyright Information: © 2016 Ana-Sosa-Batiz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funders: Australian National Health and Medical Research Council (NHMRC), Australian Government Department of Health
Projects and Grants: NHMRC 628331, NHMRC 1023294, NHMRC 510488
Date Deposited: 16 Jan 2019 07:43
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320405 Humoural immunology and immunochemistry @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320404 Cellular immunology @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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