Avian influenza rapidly induces antiviral genes in duck lung and intestine

Vanderven, Hillary A., Petkau, Kristina, Ryan-Jean, Kieran E.E., Aldridge Jr., Jerry R., Webster, Robert G., and Magor, Katharine E. (2012) Avian influenza rapidly induces antiviral genes in duck lung and intestine. Molecular Immunology, 51 (3-4). pp. 316-324.

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Abstract

Ducks are the natural reservoir of influenza A and survive infection by most strains. To characterize the duck immune response to influenza, we sought to identify innate immune genes expressed early in an infection. We used suppressive subtractive hybridization (SSH) to construct 3 libraries enriched in differentially expressed genes from lung RNA of a duck infected with highly pathogenic avian influenza virus A/Vietnam/1203/04 (H5N1), or lung and intestine RNA of a duck infected with low pathogenic avian influenza A/mallard/BC/500/05 (H5N2) compared to a mock-infected duck. Sequencing of 1687 clones identified a transcription profile enriched in genes involved in antiviral defense and other cellular processes. Major histocompatibility complex class I (MHC I), interferon induced protein with tricopeptide repeats 5 (IFIT5), and 2'-5' oligoadenylate synthetase-like gene (OASL) were increased more than 1000-fold in relative transcript abundance in duck lung at 1 dpi with highly pathogenic VN1203. These genes were induced much less in lung or intestine following infection with low pathogenic BC500. The expression of these genes following infection suggests that ducks initiate an immediate and robust response to a potentially lethal influenza strain, and a minimal response to a low pathogenic strain.

Item ID: 56885
Item Type: Article (Research - C1)
ISSN: 1872-9142
Keywords: MHC class I, Interferon stimulated genes, IFIT, OASL, Suppressive subtractive hybridization, RIG-I
Copyright Information: © 2012 Elsevier Ltd. All rights reserved.
Funders: Canadian Institute of Health Research (CIHR), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), American Lebanese Syrian Associated Charities (ALSAC)
Projects and Grants: CIHR MOP93561, NIH Department of Health and Human Services Contract No. HHS26620090005C
Date Deposited: 16 Jan 2019 07:43
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110707 Innate Immunity @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920120 Zoonoses @ 100%
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