Susceptibility of eastern water dragons Intellagama lesueurii lesueurii to Bohle iridovirus

Maclaine, Alicia, Mashkour, Narges, Scott, Jennifer, and Ariel, Ellen (2018) Susceptibility of eastern water dragons Intellagama lesueurii lesueurii to Bohle iridovirus. Diseases of Aquatic Organisms, 127. pp. 97-105.

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View at Publisher Website: https://doi.org/10.3354/dao03193
 
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Abstract

Ranaviruses infect and have been associated with mass mortality events in fish, amphibians and reptiles and are capable of interclass transmission. Eastern water dragons (EWDs), a semi-aquatic squamate, have an overlapping distribution with several species shown to be susceptible to Bohle iridovirus (BIV). However, this species has not been previously investigated, and no known mass mortalities have occurred in wild populations. Here we report the experimental infection of juvenile EWDs with BIV to investigate a water-dwelling lizards’ susceptibility to a ranaviral strain present in northern Queensland, Australia. Lizards were exposed via oral inoculation, intramuscular injection, or cohabitation with orally infected lizards. All exposure methods were effective in establishing an infection as demonstrated by skin lesions and pathological changes in the internal organs. Necrosis, haemorrhage and inflammation were observed histologically in the pancreas, liver, spleen, kidney and submucosa of the gastrointestinal tract of BIV-exposed lizards. Variably sized basophilic intracytoplasmic inclusion bodies were observed in the liver of 6/14 BIV-exposed lizards. Virus was isolated from the liver and kidney of all BIVinfected lizards and confirmed with quantitative PCR (qPCR). The outcome of this study demonstrates that juvenile EWDs are susceptible to BIV, thereby adding Australian lizards to the broad host range of ranaviruses. Furthermore, this study provides additional evidence of BIV’s ability to infect different classes of ecothermic vertebrates.

Item ID: 56615
Item Type: Article (Research - C1)
ISSN: 1616-1580
Keywords: ranavirus, Bohle iridovirus, experimental infection, reptiles, lizards
Copyright Information: © Inter-Research 2018 · www.int-res.com
Funders: Australian Postgraduate Award
Date Deposited: 16 Dec 2018 23:38
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 100%
SEO Codes: 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960406 Control of Pests, Diseases and Exotic Species in Fresh, Ground and Surface Water Environments @ 100%
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