Effect of heat processing on IgE reactivity and cross-reactivity of Tropomyosin and other allergens of Asia-Pacific mollusc species: identification of novel Sydney rock oyster Tropomyosin Sac g 1

Rolland, Jennifer M., Varese, Nirupama P., Abramovitch, Jodie B., Anania, Jessica, Nugraha, Roni, Kamath, Sandip, Hazard, Anita, Lopata, Andreas L., and O’Hehir, Robyn E. (2018) Effect of heat processing on IgE reactivity and cross-reactivity of Tropomyosin and other allergens of Asia-Pacific mollusc species: identification of novel Sydney rock oyster Tropomyosin Sac g 1. Molecular Nutrition & Food Research, 62 (14). 1800148.

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Abstract

Scope: Shellfish allergy is an increasing global health priority, frequently affecting adults. Molluscs are an important shellfish group causing food allergy but knowledge of their allergens and cross-reactivity is limited. Optimal diagnosis of mollusc allergy enabling accurate advice on food avoidance is difficult. Allergens of four frequently ingested Asia-Pacific molluscs are characterized: Sydney rock oyster (Saccostrea glomerata), blue mussel (Mytilus edulis), saucer scallop (Amusium balloti), and southern calamari (Sepioteuthis australis), examining cross-reactivity between species and with blue swimmer crab tropomyosin, Por p 1.

Methods and results: IgE ELISA showed that cooking increased IgE reactivity of mollusc extracts and basophil activation confirmed biologically relevant IgE reactivity. Immunoblotting demonstrated strong IgE reactivity of several proteins including one corresponding to heat-stable tropomyosin in all species (37–40 kDa). IgE-reactive Sydney rock oyster proteins were identified by mass spectrometry, and the novel major oyster tropomyosin allergen was cloned, sequenced, and designated Sac g 1 by the IUIS. Oyster extracts showed highest IgE cross-reactivity with other molluscs, while mussel cross-reactivity was weakest. Inhibition immunoblotting demonstrated high cross-reactivity between tropomyosins of mollusc and crustacean species.

Conclusion: These findings inform novel approaches for reliable diagnosis and improved management of mollusc allergy.

Item ID: 56035
Item Type: Article (Research - C1)
ISSN: 1613-4133
Copyright Information: © 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Funders: Australian Research Council (ARC)
Date Deposited: 06 Nov 2018 01:30
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 40%
11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics) @ 60%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 70%
92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 30%
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