The involvement of Kidney DNA methylation in blood pressure regulation

Wise, Ingrid A., Pretes, Precilla, Tomaszewski, Maciej D., and Charchar, Fadi J. (2016) The involvement of Kidney DNA methylation in blood pressure regulation. In: [Presented at the 16th Annual Conference of the Australasian Genomic Technologies Association and the 8th Annual NZ Next Generation Sequencing Conference]. From: AGTA/NZ NGS 2016: 16th Annual Conference of the Australasian Genomic Technologies Association and the 8th Annual NZ Next Generation Sequencing Conference, 9-12 October 2016, Auckland, New Zealand.

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Abstract

Background and aims: Increasing evidence suggests that epigenetic modifications such as DNA methylation (5mC) is important to the development of essential hypertension, and that changes in DNA methylation of blood cells is associated to blood pressure (BP). So far there has been no studies of epigenetic changes in the kidney - an important effector organ in BP regulation. The aim of this study was to compare the global and gene specific methylation status in the kidney between normal and hypertensive subjects.

Methods and results: We used 96 human renal tissue samples from the TRANScriptome of RenaL HumAN TissueE (TRANSLATE) Study to measure DNA methylation. TRANSLATE consists of carefully characterised collections of “apparently healthy” specimens of human kidneys. DNA was extracted from peripheral blood leukocytes and kidney tissue using the DNeasy blood and tissue Qiagen kit. Global methylation was measured by ELISA assay to determine the percentage of 5mC and loci specific methylation status was determined using Infinium HumanMethylation 450K array (Illumina®, Australia).

A significant negative relationship was found in the renal samples between 5mC% and systolic (SBP) and diastolic (DBP) blood pressure readings (SBP r=-0.25, P=<0.05), DBP r=-0.32, P=<0.01). This correlation was also evident when BP is adjusted for hypertensive medication effects (adjusted SBP P=<0.05, adjusted DBP P=<0.01). There was no significant relationship in DNA extracted from peripheral blood leukocytes between 5mC% and BP reading. We found 275 loci differentially methylated between hypertensive and normotensive individuals.

Conclusions: DNA methylation is an important molecular mechanism for BP and hypertension in humans Maciej D Tomaszewski3, Fadi J Charchar4*

Item ID: 55723
Item Type: Conference Item (Abstract / Summary)
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Also presented at the Joint Annual Scientific Meeting of the AAS, HBPRCA and AVBS, 7-10 December 2016, Hobart, TAS, Australia.

Date Deposited: 11 Dec 2018 00:03
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060404 Epigenetics (incl Genome Methylation and Epigenomics) @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060408 Genomics @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 100%
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