TCR deep sequencing of transgenic RAG-1-deficient mice reveals endogenous TCR recombination: a cause for caution

McGuire, Helen M., Watkins, Thomas S., Field, Matthew, Taylor, Sarah, Yasuyama, Nao, Farmer, Andrew, Miles, John J., and Fazekas de St. Groth, Barbara (2018) TCR deep sequencing of transgenic RAG-1-deficient mice reveals endogenous TCR recombination: a cause for caution. Immunology and Cell Biology, 96 (6). pp. 642-645.

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Abstract

The utility of T-cell receptor (TCR) transgenic mice in medical research has been considerable, with applications ranging from basic biology all the way to translational and clinical investigations. Crossing of TCR transgenic mice with either recombination-activating gene (RAG)-1 or RAG-2 knockouts is frequently used to generate mice with a monoclonal T-cell repertoire. However, low level productive TCR rearrangement has been reported in RAG-deficient mice expressing transgenic TCRs. Using deep sequencing, we set out to directly examine and quantify the presence of these endogenous TCRs. Our demonstration that functional nontransgenic TCRs are present in nonmanipulated mice has wide reaching ramifications worthy of critical consideration.

Item ID: 54879
Item Type: Article (Refereed Research - C1)
Keywords: immunological surveillance, T cells, T-cell receptor, VDJ recombination
Copyright Information: Copyright © 2018 Australasian Society for Immunology Inc.
ISSN: 1440-1711
Funders: National Health and Medical Research Council (NHMRC)
Projects and Grants: NHMRC GNT1037298, NHMRC GNT1131732
Date Deposited: 01 Aug 2018 07:47
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110703 Autoimmunity @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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