Chronic exposure of a freshwater mussel to elevated pCO(2): effects on the control of biomineralization and ion-regulatory responses

Jeffrey, Jennifer D., Hannan, Kelly D., Hasler, Caleb T., and Suski, Cory D. (2018) Chronic exposure of a freshwater mussel to elevated pCO(2): effects on the control of biomineralization and ion-regulatory responses. Environmental Toxicology and Chemistry, 37 (2). pp. 538-550.

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Abstract

Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide (pCO(2)) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28-d elevation in pCO(2) at 15 000 and 50 000 mu atm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid-base disturbances experienced after exposure to elevated pCO(2) was assessed over a 14-d recovery period. Overall, exposure to 50 000 mu atm pCO(2) had more pronounced physiological consequences compared with 15 000 mu atm pCO(2). Over the first 7 d of exposure to 50 000 mu atm pCO(2), the mRNA abundance of chitin synthase (cs), calmodulin (cam), and calmodulin-like protein (calp) were significantly affected, suggesting that shell formation and integrity may be altered during pCO(2) exposure. After the removal of the pCO(2) treatment, mussels may compensate for the acid-base and ion disturbances experienced during pCO(2) exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ca], cs, cam, calp) as well as ion regulation (na(+)-k(+)-adenosine triphosphatase [nka]) were modulated. Effects of elevated pCO(2) on heat shock protein 70 (hsp70) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of pCO(2) exposure.

Item ID: 54748
Item Type: Article (Research - C1)
ISSN: 1552-8618
Keywords: chitin synthase, Na+-K+-ATPase, calmodulin, mollusk toxicology, freshwater toxicology, benthic macroinvertebrates
Funders: US Geological Survey (USGS), US Fish and Wildlife Service (USFWS), US Department of Agriculture (USDA)
Projects and Grants: USGS G14AC00119, USFWS F-69-R, USDA ILLU-875-947
Date Deposited: 25 Jul 2018 07:51
FoR Codes: 05 ENVIRONMENTAL SCIENCES > 0501 Ecological Applications > 050101 Ecological Impacts of Climate Change @ 40%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060204 Freshwater Ecology @ 60%
SEO Codes: 96 ENVIRONMENT > 9603 Climate and Climate Change > 960399 Climate and Climate Change not elsewhere classified @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960807 Fresh, Ground and Surface Water Flora, Fauna and Biodiversity @ 50%
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