Double-layered microsphere based dual growth factor delivery system for guided bone regeneration

Xu, Chun, Xu, Jia, Xiao, Lan, Li, Zhihao, Xiao, Yin, Dargusch, Matthew, Lei, Chang, He, Yan, and Ye, Qingsong (2018) Double-layered microsphere based dual growth factor delivery system for guided bone regeneration. RSC Advances, 8 (30). pp. 16503-16512.

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Abstract

Microsphere based drug delivery systems show great advantages for tissue engineering. However, it is still a big challenge to fabricate microspheres with capability in delivering and controlled releasing multiple growth factors. In the present study, double-layered microspheres consisting of an inner-layer of small core particles and an outer-layer of big shell particles were developed to sequentially release cell homing factors (SDF-1) and osteoinductive growth factors (BMP-2) for bone regeneration. In vitro release testing showed that bioactivity of both growth factors retained within the microspheres and differential release of SDF-1 and BMP-2 was achieved. Microspheres with both growth factors showed an obvious chemotaxis effect on preosteoblasts by inducing more cell migration. In osteoinductive ability tests, the microspheres with both growth factors showed higher ALP activity and more mineralized modules than control groups after culturing for 2 weeks. The expression of bone development transcription factors (Runx2, OCN, Osterix) as well as Smad signals (Smad 1, 5, 8) showed higher gene expression in the dual growth factor group. Our results suggest that a double-layered microsphere system enhances the recruitment of osteogenic cells and osteoinduction, which provides a promising platform for bone regeneration.

Item ID: 54429
Item Type: Article (Research - C1)
ISSN: 2046-2069
Copyright Information: This journal is © The Royal Society of Chemistry 2018. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Funders: Australian Research Council (ARC), National Natural Science Foundation of China (NSFC), National Health and Medical Research Council (NHMRC), Women's Academic Fund (WAF), Advance Queensland
Projects and Grants: ARC DE120101666, NSFC 81701032, NHMRC Early Career Fellowship, Advance Queensland, WAF WAF-4425969-189
Date Deposited: 04 Jul 2018 07:46
FoR Codes: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030405 Molecular Medicine @ 100%
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