Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bovine semen

Annandale, C. Henry, Smuts, Mario P., Ebersohn, Karen, Du Plessis, Lizette, Venter, Estelle H., and Stout, Tom (2018) Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bovine semen. Animal Reproduction Science, 195. pp. 24-29.

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Abstract

Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control sample (C), a sample spiked with a 4 log TCID50 dose of an LSDV isolate (HD) and a 103 dilution of the virus suspension (ND) and frozen routinely. Two straws from each of the different semen treatment groups (HD, ND and C) were subsequently thawed and subjected to swim-up, single layer centrifugation, Percoll® density gradient and a Percoll® density gradient with added trypsin. For one set of straws, semen quality variables were recorded, and viral DNA status determined using PCR; the other set was used for positive staining electron microscopy. Samples determined to be positive for LSDV DNA by PCR were then subjected to virus isolation (VI). Complete elimination of LSDV from semen did not occur with use of any of the processing methods. Trypsin did reduce the viral load, and eliminated LSDV from the ND sample, but severely negatively influenced semen quality. The LSDV virions, as assessed by electron microscopy, were associated with the sperm plasma membrane. Further investigation is needed to establish the efficacy of immuno-extenders for rendering semen free from LSDV.

Item ID: 54402
Item Type: Article (Research - C1)
ISSN: 1873-2232
Keywords: lumpy skin disease virus; bull; semen; sanitation methods
Funders: Higher Education and Training, South Africa
Date Deposited: 03 Jul 2018 05:01
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 80%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070799 Veterinary Sciences not elsewhere classified @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%
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