T regulatory cells disrupt the CCL20-CCR6 axis driving Th17 homing to the gut

Loiseau, Claire, Requena, Mary, Cazabat, Michelle, Carrere, Nicolas, Suc, Bertrand, Marchou, Bruno, Izopet, Jaques, and Delobel, Pierre (2015) T regulatory cells disrupt the CCL20-CCR6 axis driving Th17 homing to the gut. In: Abstracts from the 2015 Conference on Retroviruses and Opportunistic Infections. 274. p. 235. From: CROI 2015: Conference on Retroviruses and Opportunistic Infections, 23-26 February 2015, Seattle, WA, USA.

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Abstract

Background: During HIV-1 infection, the integrity of the intestinal immune barrier is disrupted due to a deep depletion of CD4 + T cells in the gut. The translocation of microbial products from the gut lumen into the bloodstream has been linked with systemic inflammation. Despite long-term effective cART, CD4 + T cells in the lamina propria are incompletely restored in most individuals.

Aims: Among the chemotactic axes involved in CD4 + T cell homing to the gut, we focused on the CCR6-CCL20 axis as it governs Th17 cells homing, a T cell subset exerting a major role in antimicrobial immunity. We aimed to assess the factors regulating the expression of CCL20 by the enterocytes, and notably the role of the cytokines produced by Treg and Th17 cells.

Methods: Small bowel biopsies were obtained by endoscopy in 20 HIV-1 + and 10 HIV-1-individuals. Intestinal lymphocytes phenotype was analyzed by flow cytometry. CCL20 mRNA was quantified by qRT-PCR. The effect of PRR ligands and cytokines on CCL20 expression was explored using an ex-vivosystem of human primary enterocytes. A coculture was done between the enterocytes and Th17/Treg cells. The expression of CCL20 by the enterocytes was evaluated by qRT-PCR and ELISA.

Item ID: 54364
Item Type: Conference Item (Poster)
ISBN: 978-0-692-36902-9
Related URLs:
Date Deposited: 28 Jun 2018 00:23
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 50%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
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