A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma

Laohaviroj, Marut, Potriquet, Jeremy, Jia, Xinying, Suttiprapa, Sutas, Chamgramol, Yaovalux, Pairojkul, Chawalit, Sithithaworn, Paiboon, Mulvenna, Jason, and Sripa, Banchob (2017) A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma. Tumor Biology, 39 (6).

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Cholangiocarcinoma is a primary malignant tumor of the bile duct epithelium. Cholangiocarcinoma is usually detected at an advanced stage when successful treatment is no longer possible. As the tumor originates from the bile duct epithelium, bile is an ideal source of tumor biomarkers for cholangiocarcinoma. In this study, we used a quantitative proteomics approach to identify potential tumor-associated proteins in the bile fluid of six cholangiocarcinoma patients. Three different gross-appearance tumor types were used in the analysis: mass-forming type (n = 2), periductal infiltrating type (n = 2), and intraductal growth type (n = 2). Two bile samples from non-cancerous patients were used as controls. Isobaric labeling, coupled with Tandem mass spectrometry, was used to quantify protein levels in the bile of cholangiocarcinoma and control patients. In all, 63 proteins were significantly increased in cholangiocarcinoma bile compared to normal bile. Alpha-1-antitrypsin was one of the overexpressed proteins that increased in cholangiocarcinoma bile samples. Immunohistochemical analysis revealed that alpha-1-antitrypsin was detected in 177 (50%) of 354 cholangiocarcinoma tissues from our Tissue Bank. Immunoblotting of 54 cholangiocarcinoma bile samples showed that alpha-1-antitrypsin was positive in 38 (70%) samples. Fecal enzyme-linked immunosorbent assay showed that alpha-1-antitrypsin level was able to distinguish cholangiocarcinoma patients from normal individuals. In conclusion, alpha-1-antitrypsin is a potential marker for early diagnosis of cholangiocarcinoma.

Item ID: 54241
Item Type: Article (Research - C1)
ISSN: 1423-0380
Keywords: Cholangiocarcinoma, biomarkers, bile, proteomics, alpha-1-antitrypsin, feces
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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

Funders: Thailand Research Fund (TRF), National Institute of Allergy and Infectious Disease (NIAID), National Cancer Institute (NCI), National Institute of Health (NIH), USA, National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: TRF grant no. RTA 5680006, NIAD grant P50AI098639, NIH award CA164719, NHMRC grant 1051627
Date Deposited: 21 Jun 2018 00:45
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320799 Medical microbiology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 100%
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