Enzyme-mediated biodegradation of long-chain n-alkanes (C₃₂ and C₄₀) by thermophilic bacteria

Elumalai, Punniyakotti, Parthipan, Punniyakotti, Obulisamy, Karthikeyan Parthiba, and Rajasekar, Aruliah (2017) Enzyme-mediated biodegradation of long-chain n-alkanes (C₃₂ and C₄₀) by thermophilic bacteria. 3 Biotech, 7. 116.

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Abstract

Removal of long-chain hydrocarbons and n-alkanes from oil-contaminated environments are mere important to reduce the ecological damages, while bio-augmentation is a very promising technology that requires highly efficient microbes. In present study, the efficiency of pure isolates, i.e., Geobacillus thermoparaffinivorans IR2, Geobacillus stearothermophillus IR4 and Bacillus licheniformis MN6 and mixed consortium on degradation of long-chain n-alkanes C 32 and C 40 was investigated by batch cultivation test. Biodegradation efficiencies were found high for C 32 by mixed consortium (90%) than pure strains, while the pure strains were better in degradation of C 40 than mixed consortium (87%). In contrast, the maximum alkane hydroxylase activities (161 µmol mg −1 protein) were recorded in mixed consortium system that had supplied with C 40 as sole carbon source. Also, the alcohol dehydrogenase (71 µmol mg −1 protein) and lipase activity (57 µmol mg −1 protein) were found high. Along with the enzyme activities, the hydrophobicity natures of the bacterial strains were found to determine the degradation efficiency of the hydrocarbons. Thus, the study suggested that the hydrophobicity of the bacteria is a critical parameter to understand the biodegradation of n-alkanes.

Item ID: 53994
Item Type: Article (Research - C1)
ISSN: 2190-572X
Keywords: Bacillus and Geobacillus; biodegradation; degradation enzymes; gas chromatography; hydrophobicity; n-Alkanes
Funders: Indian Government, Department of Biotechnology (DOB), Indian Government, Department of Science and Technology (DOST), University Grants Commission (MRP)
Projects and Grants: DOB re-entry fellowship BT/RLF/Re-entry/17/2012, DOST young scientist award SB/YS/LS-40/2013, MRP-MAJOR-MICRO-2013-31825, DOST Science and Engineering Research Board EEQ/2016/000449
Date Deposited: 12 Jun 2018 03:56
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 50%
05 ENVIRONMENTAL SCIENCES > 0599 Other Environmental Sciences > 059999 Environmental Sciences not elsewhere classified @ 50%
SEO Codes: 96 ENVIRONMENT > 9612 Rehabilitation of Degraded Environments > 961299 Rehabilitation of Degraded Environments not elsewhere classified @ 100%
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