In vitro response of rumen microbiota to the antimethanogenic red acroalga Asparagopsis taxiformis

Machado, Lorenna, Tomkins, Nigel, Magnusson, Marie, Midgley, David J., de Nys, Rocky, and Rosewarne, Carly P. (2018) In vitro response of rumen microbiota to the antimethanogenic red acroalga Asparagopsis taxiformis. Microbial Ecology, 75 (3). pp. 811-818.

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Abstract

The red macroalga Asparagopsis taxiformis has been shown to significantly decrease methane production by rumen microbial communities. This has been attributed to the bioaccumulation of halogenated methane analogues produced as algal secondary metabolites. The objective of this study was to evaluate the impact of A. taxiformis supplementation on the relative abundance of methanogens and microbial community structure during in vitro batch fermentation. Addition of A. taxiformis (2% organic matter) or the halogenated methane analogue bromoform (5 μM) reduced methane production by over 99% compared to a basal substrate-only control. Quantitative PCR confirmed that the decrease in methane production was correlated with a decrease in the relative abundance of methanogens. High-throughput 16S ribosomal RNA gene amplicon sequencing showed that both treatments reduced the abundance of the three main orders of methanogens present in ruminants (Methanobacteriales, Methanomassiliicoccales and Methanomicrobiales). Shifts in bacterial community structure due to the addition of A. taxiformis and 5 μM bromoform were similar and concomitant with increases in hydrogen concentration in the headspace of the fermenters. With high potency and broad-spectrum activity against rumen methanogens, A. taxiformis represents a promising natural strategy for reducing enteric methane emissions from ruminant livestock.

Item ID: 53808
Item Type: Article (Research - C1)
ISSN: 1432-184X
Keywords: methane; rumen; livestock; seaweed; bromoform
Funders: Department of Agriculture, Meat and Livestock Australia, MBD Energy, Advanced Manufacturing CRC
Date Deposited: 26 Jul 2018 02:46
FoR Codes: 10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8398 Environmentally Sustainable Animal Production > 839802 Management of Greenhouse Gas Emissions from Animal Production @ 100%
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