Estimation of the uncertainty of analyte concentration from the measurement uncertainty

Brown, Simon, Cooke, Delwyn G., and Blackwell, Leonard F. (2015) Estimation of the uncertainty of analyte concentration from the measurement uncertainty. Scandinavian Journal of Clinical & Laboratory Investigation, 75 (5). pp. 367-373.

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Abstract

Ligand-binding assays, such as immunoassays, are usually analysed using standard curves based on the four-parameter and five-parameter logistic models. An estimate of the uncertainty of an analyte concentration obtained from such curves is needed for confidence intervals or precision profiles. Using a numerical simulation approach, it is shown that the uncertainty of the analyte concentration estimate becomes significant at the extremes of the concentration range and that this is affected significantly by the steepness of the standard curve. We also provide expressions for the coefficient of variation of the analyte concentration estimate from which confidence intervals and the precision profile can be obtained. Using three examples, we show that the expressions perform well.

Item ID: 53003
Item Type: Article (Research - C1)
ISSN: 1502-7686
Keywords: analytical chemistry methods; clinical chemistry tests; immunoassay; logistic models; uncertainty
Date Deposited: 05 Apr 2018 23:22
FoR Codes: 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030106 Quality Assurance, Chemometrics, Traceability and Metrological Chemistry @ 60%
03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030104 Immunological and Bioassay Methods @ 40%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 100%
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