Molecular evidence for the localization of plasmodium falciparum immature gametocytes in bone marrow

Aguilar, Ruth, Magallon-Tejada, Ariel, Achtman, Ariel, Moraleda, Cinta, Joice, Regina, Cisteró, Pau, Li Wai Suen, Connie S.N., Nhabomba, Augusto, Macete, Eusebio, Mueller, Ivo, Marti, Matthias, Alonso, Pedro L., Menéndez, Clara, Schofield, Louis, and Mayor, Alfredo (2014) Molecular evidence for the localization of plasmodium falciparum immature gametocytes in bone marrow. Blood, 123 (7). pp. 959-966.

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Abstract

Plasmodium falciparum immature gametocytes are not observed in peripheral blood. However, gametocyte stages in organs such as bone marrow have never been assessed bymolecular techniques, which are more sensitive than optical microscopy. We quantified P falciparum sexual stages in bone marrow (n = 174) and peripheral blood (n = 70) of Mozambican anemic children by quantitative polymerase chain reaction targeting transcripts specific for early (PF14-0748; PHISTa), intermediate (PF13-0247; Pfs48/45), and mature (PF10-0303; Pfs25) gametocytes. Among children positive for the P falciparum housekeeping gene (PF08-0085; ubiquitin-conjugating enzyme gene) in bone marrow (n = 136) and peripheral blood (n = 25), prevalence of immature gametocytes was higher in bone marrow than peripheral blood (early: 95% vs 20%, P < .001; intermediate: 80% vs 16%; P < .001), as were transcript levels (P < .001 for both stages). In contrast, mature gametocytes were more prevalent (100% vs 51%, P < .001) and abundant (P < .001) in peripheral blood than in the bone marrow. Severe anemia (3.57, 95% confidence interval 1.49-8.53) and dyserythropoiesis (6.21, 95% confidence interval 2.24-17.25) were independently associated with a higher prevalence of mature gametocytes in bone marrow. Our results highlight the high prevalence and abundance of early sexual stages in bone marrow, aswell as the relationship between hematological disturbances and gametocyte development in this tissue. © 2014 by The American Society of Hematology.

Item ID: 52615
Item Type: Article (Research - C1)
ISSN: 1528-0020
Funders: Agencia de Cooperacion Internacional de Las Illes Balears, Fundación Ramón Areces, Bill and Melinda Gates Foundation (BMGF), Spanish Agency for International Cooperation and Development, Victorian State Government Operational Infrastructure Support, National Health and Medical Research Council (NHMRC), Tecnologia e Innovacion de la Republica de Panama, National Institute of Allergy and Infectious Disease (NIAID), Instituto de Salud Carlos III (ISC)
Projects and Grants: BMGF Malaria Transmission Epidemiology Project, NHMRC Independent Research Institutes Infrastructure Support Scheme, NIAID (R01A107755801), ISC CP-04/00220
Date Deposited: 20 Feb 2018 23:57
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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