Revisiting the Ancylostoma caninum secretome provides new information on hookworm-host interactions

Morante, Taylor, Shepherd, Catherine, Constantinoiu, Constantin, Loukas, Alex, and Sotillo, Javier (2017) Revisiting the Ancylostoma caninum secretome provides new information on hookworm-host interactions. Proteomics, 17 (23-24). 1700176.

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Hookworm infection is a major tropical parasitic disease affecting almost 500 million people worldwide. These soil-transmitted helminths can survive for many years in the intestine of the host, where they feed on blood, causing iron deficiency anemia and other complications. These parasites release a variety of molecules known as excretory/secretory products (ESPs) that are involved in many different biological processes that govern parasite survival. Using a combination of separation techniques such as SDS-PAGE and OFFGEL electrophoresis, in combination with state-of-the-art mass spectrometry we have reanalyzed the dog hookworm, Ancylostoma caninum, ESPs (AcESP). We identified 315 proteins present in the AcESP, compared with just 105 identified in previous studies. The most highly represented family of proteins is the SCP/TAPs (110 of the 315 proteins), and the most abundant constituents of AcESP are homologues of the tissue inhibitors of metalloproteases (TIMP) family. Interestingly, we identified new homologs of well-known vaccine candidates and immunomodulatory proteins. This study provides novel information about the proteins secreted by A. caninum, and constitutes a comprehensive dataset to study the proteins involved in host-hookworm interactions.

Item ID: 51943
Item Type: Article (Research - C1)
ISSN: 1615-9861
Keywords: Ancylostoma caninum, excretory, hookworm, proteomics, secretome, secretory products
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC grant no. 1037304, NHMRC Senior Research Fellowship 1117504
Date Deposited: 10 Jan 2018 07:41
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 100%
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