Confirmation of stormwater bioretention treatment effectiveness using molecular indicators of cardiovascular toxicity in developing fish

McIntyre, Jenifer K., Edmunds, Richard C., Redig, Maria G., Mudrock, Emma M., Davis, Jay W., Incardona, John P., Stark, John D., and Scholz, Nathaniel L. (2016) Confirmation of stormwater bioretention treatment effectiveness using molecular indicators of cardiovascular toxicity in developing fish. Environmental Science and Technology, 50 (3). pp. 1561-1569.

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Abstract

Urban stormwater runoff is a globally significant threat to the ecological integrity of aquatic habitats. Green stormwater infrastructure methods such as bioretention are increasingly used to improve water quality by filtering chemical contaminants that may be harmful to fish and other species. Ubiquitous examples of toxics in runoff from highways and other impervious surfaces include polycyclic aromatic hydrocarbons (PAHs). Certain PAHs are known to cause functional and structural defects in developing fish hearts. Therefore, abnormal heart development in fish can be a sensitive measure of clean water technology effectiveness. Here we use the zebrafish experimental model to assess the effects of untreated runoff on the expression of genes that are classically responsive to contaminant exposures, as well as heart-related genes that may underpin the familiar cardiotoxicity phenotype. Further, we assess the effectiveness of soil bioretention for treating runoff, as measured by prevention of both visible cardiac toxicity and corresponding gene regulation. We find that contaminants in the dissolved phase of runoff (e.g., PAHs) are cardiotoxic and that soil bioretention protects against these harmful effects. Molecular markers were more sensitive than visible toxicity indicators, and several cardiac-related genes show promise as novel tools for evaluating the effectiveness of evolving stormwater mitigation strategies.

Item ID: 51384
Item Type: Article (Research - C1)
ISSN: 1520-5851
Funders: Environmental Protection Agency (EPA), National Oceanic and Atmospheric Administration (NOAA), National Ocean Services, U.S. Fish and Wildlife Service
Projects and Grants: EPA DW-14-95791701-1, NOAA Award No. NA14OAR4170078
Date Deposited: 01 Nov 2017 23:10
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 50%
05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050204 Environmental Impact Assessment @ 50%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960511 Ecosystem Assessment and Management of Urban and Industrial Environments @ 50%
96 ENVIRONMENT > 9611 Physical and Chemical Conditions of Water > 961101 Physical and Chemical Conditions of Water for Urban and Industrial Use @ 50%
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