Corresponding morphological and molecular indicators of crude oil toxicity to the developing hearts of mahi mahi

Edmunds, Richard C, Gill, J.A., Baldwin, David H., Linbo, Tiffany L., French, Barbara L., Brown, Tanya L., Esbaugh, Andrew J., Mager, Edward M., Stieglitz, John, Hoenig, Ron, Benetti, Daniel, Grosell, Martin, Scholz, Nathaniel L., and Incardona, John P. (2015) Corresponding morphological and molecular indicators of crude oil toxicity to the developing hearts of mahi mahi. Scientific Reports, 5. 17326.

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Abstract

Crude oils from distinct geological sources worldwide are toxic to developing fish hearts. When oil spills occur in fish spawning habitats, natural resource injury assessments often rely on conventional morphometric analyses of heart form and function. The extent to which visible indicators correspond to molecular markers for cardiovascular stress is unknown for pelagic predators from the Gulf of Mexico. Here we exposed mahi (Coryphaena hippurus) embryos to field-collected crude oil samples from the 2010 Deepwater Horizon disaster. We compared visible heart defects (edema, abnormal looping, reduced contractility) to changes in expression of cardiac-specific genes that are diagnostic of heart failure in humans or associated with loss-of-function zebrafish cardiac mutants. Mahi exposed to crude oil during embryogenesis displayed typical symptoms of cardiogenic syndrome as larvae. Contractility, looping, and circulatory defects were evident, but larval mahi did not exhibit downstream craniofacial and body axis abnormalities. A gradation of oil exposures yielded concentration-responsive changes in morphometric and molecular responses, with relative sensitivity being influenced by age. Our findings suggest that 1) morphometric analyses of cardiac function are more sensitive to proximal effects of crude oil-derived chemicals on the developing heart, and 2) molecular indicators reveal a longer-term adverse shift in cardiogenesis trajectory.

Item ID: 51380
Item Type: Article (Research - C1)
ISSN: 2045-2322
Additional Information:

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Funders: Deep Water Horizon (DWH)
Projects and Grants: DWH Natural Resource Damage Assessment
Date Deposited: 01 Nov 2017 23:42
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 50%
05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050204 Environmental Impact Assessment @ 50%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960506 Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments @ 50%
96 ENVIRONMENT > 9611 Physical and Chemical Conditions of Water > 961104 Physical and Chemical Conditions of Water in Marine Environments @ 50%
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