Comparative genomics of Burkholderia singularis sp. nov., a low GCC content, free-living bacterium that defies taxonomic dissection of the genus Burkholderia

Vandamme, Peter, Peeters, Charlotte, De Smet, Birgit, Price, Erin P., Sarovich, Derek S., Henry, Deborah A., Hird, Trevor J., Zlosnik, James E.A., Mayo, Mark, Warner, Jeffrey, Baker, Anthony, Currie, Bart J., and Carlier, Aurélien (2017) Comparative genomics of Burkholderia singularis sp. nov., a low GCC content, free-living bacterium that defies taxonomic dissection of the genus Burkholderia. Frontiers In Microbiology, 8. 1679.

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Abstract

Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. The type strain is LMG 28154T (=CCUG 65685T). Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of other Burkholderia species. The reduced G+C content of strain LMG 28154T was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents.

Item ID: 51296
Item Type: Article (Research - C1)
ISSN: 1664-302X
Keywords: Burkholderia singularis; whole genome sequence; cystic fibrosis microbiology; comparative genomics; Burkholderia pseudomallei complex; Burkholderia cepacia complex
Additional Information:

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Funders: Australian National Health and Medical Research Council (NHMRC)
Projects and Grants: NHMRC Project Grant 1098337, NHMRC Project Grant 1131932
Date Deposited: 23 Oct 2017 01:41
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 100%
SEO Codes: 92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 100%
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