Surface immuno-functionalisation for the capture and detection of vibrio species in the marine environment: a new management tool for industrial facilities

Laczka, Olivier F., Labbate, Maurizio, Seymour, Justin R., Bourne, David G., Fielder, Stewart S., and Doblin, Martina A. (2014) Surface immuno-functionalisation for the capture and detection of vibrio species in the marine environment: a new management tool for industrial facilities. PLoS One, 9 (10).

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Abstract

Bacteria from the genus Vibrio are a common and environmentally important group of bacteria within coastal environments and include species pathogenic to aquaculture organisms. Their distribution and abundance are linked to specific environmental parameters, including temperature, salinity and nutrient enrichment. Accurate and efficient detection of Vibrios in environmental samples provides a potential important indicator of overall ecosystem health while also allowing rapid management responses for species pathogenic to humans or species implicated in disease of economically important aquacultured fish and invertebrates. In this study, we developed a surface immuno-functionalisation protocol, based on an avidin-biotin type covalent binding strategy, allowing specific sandwich-type detection of bacteria from the Vibrio genus. The assay was optimized on 12 diverse Vibrio strains, including species that have implications for aquaculture industries, reaching detection limits between 7x10(3) to 3x10(4) cells mL(-1). Current techniques for the detection of total Vibrios rely on laborious or inefficient analyses resulting in delayed management decisions. This work represents a novel approach for a rapid, accurate, sensitive and robust tool for quantifying Vibrios directly in industrial systems and in the environment, thereby facilitating rapid management responses.

Item ID: 51144
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

© 2014 Laczka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: University of Technology, Sydney (UTS), Transfield Foundation
Projects and Grants: UTS Chancellor's Postdoctoral Fellowship Grant number: 2012000115
Date Deposited: 11 Oct 2017 07:43
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%
SEO Codes: 96 ENVIRONMENT > 9699 Other Environment > 969999 Environment not elsewhere classified @ 100%
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