Anti-mycobacterial function of macrophages is impaired in a diet induced model of type 2 diabetes

Alim, Md Abdul, Sikder, Suchandan, Bridson, Tahnee L., Rush, Catherine M., Govan, Brenda L., and Ketheesan, Natkunam (2017) Anti-mycobacterial function of macrophages is impaired in a diet induced model of type 2 diabetes. Tuberculosis, 102. pp. 47-54.

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Type 2 diabetes (T2D) is one of the major risk factors for tuberculosis (TB). In this study, a diet induced murine model of T2D (DIMT2D) was developed and characterized in the context of metabolic, biochemical and histopathological features following diet intervention. Mycobacterial susceptibility was investigated using Mycobacterium fortuitum as a surrogate. Phagocytic capability of alveolar macrophages and resident peritoneal macrophages were determined by in vitro assays using mycolic acid coated beads and M. fortuitum. Results demonstrated that bacillary loads were significantly higher in liver, spleen, and lungs of diabetic mice compared to controls. Higher inflammatory lesions and impaired cytokine kinetics (TNF-alpha, MCP-1, IL-12, IFN-gamma) were also observed in diabetic mice. Macrophages isolated from diabetic mice had lower uptake of mycolic acid coated beads, reduced bacterial internalization and killing and altered cytokine responses (TNF-alpha, IL-6, MCP-1). This model will be useful to further investigate different facets of host-pathogen interactions in TB-T2D.

Item ID: 50680
Item Type: Article (Research - C1)
ISSN: 1873-281X
Keywords: murine model, macrophage, tuberculosis, type 2 diabetes, phagocytosis
Funders: James Cook University
Date Deposited: 20 Sep 2017 11:01
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320702 Medical infection agents (incl. prions) @ 20%
42 HEALTH SCIENCES > 4202 Epidemiology > 420207 Major global burdens of disease @ 80%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920104 Diabetes @ 100%
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