3% NaCl adenosine, lidocaine, Mg2+ (ALM) bolus and 4 hours "drip" infusion reduces noncompressible hemorrhage by 60% in a rat model

Letson, Hayley, and Dobson, Geoffrey (2017) 3% NaCl adenosine, lidocaine, Mg2+ (ALM) bolus and 4 hours "drip" infusion reduces noncompressible hemorrhage by 60% in a rat model. Journal of Trauma and Acute Care Surgery, 82 (6). pp. 1063-1072.

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Abstract

Background: Noncompressible torso hemorrhage is the leading cause of potentially survivable trauma in far-forward combat environments. Our aim was to examine the effect of small-volume 3% NaCl adenosine, lidocaine, and Mg (ALM) bolus and 0.9% NaCl/ALM "drip" on survivability and cardiac/gut/kidney function in a rat model of hepatic hemorrhage and shock.

Methods: Male Sprague-Dawley rats (428 ± 4 g) were anesthetized and randomly assigned to one of five groups (n = 16): (1) Sham, (2) No treatment, (3) Saline controls, (4) ALM therapy, and (5) Hextend. Animals were ventilated, instrumented with single or double laparotomy for tissue probe insertion, and hemorrhage induced by liver resection. After 15 minutes, a single 3% NaCl ± ALM bolus (0.7 ml/kg) was injected IV (phase 1) and after 60 minutes, 4 hours 0.9% NaCl ± ALM stabilization "drip" (0.5 ml/kg/h) was administered (phase 2), with 1-hour monitoring.

Results: Mortality for Shams (no resection) was 0% (25%); No treatment, 87.5% (100%); Saline controls, 37.5% (75%); ALM therapy, 0% (25%), and Hextend, 87.5% (100%) (double laparotomy in parentheses). Hextend-treated animals died during the first 20 minutes of phase 2. A single ALM bolus during phase 1 led to a 2.4-fold higher cardiac output and improved hemodynamics. 3% NaCl ALM bolus increased tissue pO2 and flow in gut and kidney during phase 1 and, during ALM "drip" in phase 2, tissue pO2 decreased but flow continued to rise, indicating increased tissue O2 extraction and delivery. During phase 2, CO, ejection fraction, and fractional shortening decreased and were erratic in all groups except ALM treatment. ALM therapy led to up to 60% less bleeding over 6 hours compared to Saline controls and 75% less bleeding than Hextend.

Conclusions: Small-volume ALM therapy significantly reduced mortality and internal bleeding compared to Saline controls or Hextend-treated rats. Hextend increased mortality, severe bleeding, and microvascular-organ injury.

Item ID: 50173
Item Type: Article (Research - C1)
ISSN: 2163-0763
Keywords: noncompressible hemorrhage; ALS; survival; far forward; hypotensive resuscitation
Funders: Department of Defence (DoD), USA, James Cook University
Research Data: http://dx.doi.org/10.4225/28/59c446100467b
Date Deposited: 13 Sep 2017 02:38
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiovascular Medicine and Haematology > 110299 Cardiovascular Medicine and Haematology not elsewhere classified @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 40%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 60%
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