Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct
Burel, Julie G., Apte, Simon H., Groves, Penny L., McCarthy, James S., and Doolan, Denise L. (2017) Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct. JCI Insight, 2 (3). e87499. pp. 1-12.
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Abstract
Pathogen-specific polyfunctional T cell responses have been associated with favorable clinical outcomes, but it is not known whether molecular differences exist between polyfunctional and monofunctional cytokine-producing T cells. Here, we report that polyfunctional CD4+ T cells induced during Plasmodium falciparum (P. falciparum) blood-stage infection in humans have a unique transcriptomic profile compared with IFN-γ monofunctional CD4+ T cells and, thus, are molecularly distinct. The 14-gene signature revealed in P. falciparum–reactive polyfunctional T cells is associated with cytokine signaling and lymphocyte chemotaxis, and systems biology analysis identified IL-27 as an upstream regulator of the polyfunctional gene signature. Importantly, the polyfunctional gene signature is largely conserved in Influenza-reactive polyfunctional CD4+ T cells, suggesting that polyfunctional T cells have core characteristics independent of pathogen specificity. This study provides the first evidence to our knowledge that consistent molecular differences exist between polyfunctional and monofunctional CD4+ T cells.
Item ID: | 50070 |
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Item Type: | Article (Research - C1) |
ISSN: | 2379-3708 |
Funders: | National Health and Medical Research Council (NHMRC), University of Queensland, Queensland Government |
Projects and Grants: | NHMRC Project grant 1037304 |
Date Deposited: | 05 Sep 2017 02:13 |
FoR Codes: | 42 HEALTH SCIENCES > 4206 Public health > 420605 Preventative health care @ 50% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320404 Cellular immunology @ 25% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320405 Humoural immunology and immunochemistry @ 25% |
SEO Codes: | 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100% |
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