Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Jenkins, A.O., Cadmus, S.I.B., Venter, E.H., Pourcel, C., Hauk, Y., Vergnaud, G., and Godfroid, J. (2011) Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria. Veterinary Microbiology, 151 (1-2). pp. 139-147.

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Abstract

From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1–18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19–48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans.

Item ID: 49052
Item Type: Article (Research - C1)
ISSN: 1873-2542
Keywords: Mycobacterium tuberculosis complex, spoligotyping, MLVA, zoonoses, Nigeria
Funders: University of Pretoria, Institute of Tropical Medicine, Belgium, University of Ibadan (UI)
Projects and Grants: UI Re-entry Grant 2006
Date Deposited: 04 Jul 2017 22:52
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%
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