Diet-tissue discrimination factors and turnover of carbon and nitrogen stable isotopes in tissues of an adult predatory coral reef fish, Plectropomus leopardus

Matley, J.K., Fisk, A.T., Tobin, A.J., Heupel, M.R., and Simpfendorfer, C.A. (2016) Diet-tissue discrimination factors and turnover of carbon and nitrogen stable isotopes in tissues of an adult predatory coral reef fish, Plectropomus leopardus. Rapid Communications in Mass Spectrometry, 30 (1). pp. 29-44.

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Abstract

Rationale: Stable isotope ratios (δ13C and δ15N values) provide a unique perspective into the ecology of animals because the isotope ratio values of consumers reflect the values in food. Despite the value of stable isotopes in ecological studies, the lack of species-specific experimentally derived diet-tissue discrimination factors (DTDFs) and turnover rates limits their application at a broad scale. Furthermore, most aquatic feeding experiments use temperate, fast-growing fish species and few have considered medium- to large-sized adults with low growth rates from tropical ecosystems.

Methods: A controlled-diet stable isotope feeding trial was conducted over a 196-day period for the adult predatory reef fish leopard coralgrouper (Plectropomus leopardus). This study calculated δ13C and δ15N DTDFs and turnover rates in five tissues (liver, plasma, red blood cells (RBC), fin, and muscle) using a continuous flow isotope ratio mass spectrometer equipped with an elemental analyzer. In addition, the effect of chemical lipid extraction (LE) on stable isotope values was examined for each tissue.

Results: Turnover was mainly influenced by metabolism (as opposed to growth) with LE δ15N half-life values lowest in fin (37 days) and plasma (66 days), and highest in RBC (88 days) and muscle (126 days). The diet-tissue discrimination factors for δ15N values in all tissues (Δ15N: –0.15 to 1.84‰) were typically lower than commonly reported literature values. Lipid extraction altered both δ15N and δ13C values compared with untreated samples; however, for the δ15N values, the differences were small (mean δ15NLE-Bulk <0.46‰ in all tissues).

Conclusions: This study informs future interpretation of stable isotope data for medium- to large-sized fish and demonstrates that DTDFs developed for temperate fish species, particularly for δ15N values, may not apply to tropical species. Sampling of muscle and/or RBC is recommended for a relatively long-term representation of feeding habits, while plasma and/or fin should be used for a more recent indication of diet.

Item ID: 48507
Item Type: Article (Research - C1)
ISSN: 1097-0231
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Additional Information:

A version of this publication was included as Chapter 3 of the following PhD thesis: Matley, Jordan Kyle (2017) Investigation of coral trout (Plectropomus spp.) movement patterns and resource use: a multidisciplinary approach using acoustic telemetry and dietary indicators. PhD thesis, James Cook University, which is available Open Access in ResearchOnline@JCU. Please see the Related URLs for access.

Funders: Australian Research Council (ARC), Australian Institute of Marine Science (AIMS), James Cook University (JCU), Australian Coral Reef Society (ACRS)
Projects and Grants: ARC FT100101004
Date Deposited: 12 Apr 2017 05:39
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070403 Fisheries Management @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070402 Aquatic Ecosystem Studies and Stock Assessment @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830299 Fisheries- Wild Caught not elsewhere classified @ 100%
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