An improved method for determining virucidal efficacy of a chemical disinfectant using an electrical impedance assay

Ebersohn, Karen, Coetzee, Peter, and Venter, E.H. (2014) An improved method for determining virucidal efficacy of a chemical disinfectant using an electrical impedance assay. Journal of Virological Methods, 199. pp. 25-28.

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Abstract

A major problem with the testing of virucidal efficacy using current protocols is that scoring of virus-induced cytopathic effect (CPE) is dependent on subjective visual interpretation using light microscopy. The current report details the use of an electrical impedance assay (xCELLigence, ACEA Biosciences) for its utility in virucidal efficacy testing. In this study, the xCELLigence system was used in a procedure developed from guidelines given by the Deutsche Vereiniging zur Bekampfung der Viruskrankheiten (DVV) (German Association for the Control of Virus Diseases) in order to demonstrate the inactivation of infectious bursal disease virus using a commercial virucide. Although the modified DVV assay using the xCELLigence system yielded identical results (i.e. a 5-log 10 reduction in viral infectivity) as the traditional DVV assay, the system allows virucidal efficacy and cytotoxicity to be measured in a more precise and reproducible fashion.

Item ID: 47830
Item Type: Article (Research - C1)
ISSN: 1879-0984
Keywords: electrical impedance assay, virucide efficacy, infectious bursal disease virus, cytopathic effect
Date Deposited: 15 Mar 2017 07:38
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%
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