Glazov, Evgeny A., Horwood, Paul F., Assavalapsakul, Wanchai, Kongsuwan, Kritaya, Mitchell, Roger W., Mitter, Neena, and Mahony, Timothy J. (2010) Characterization of microRNAs encoded by the bovine herpesvirus 1 genome. Journal of General Virology, 91 (1). pp. 32-41.

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Abstract

Bovine herpesvirus 1 (BoHV-1) is a ubiquitous and important pathogen of cattle worldwide. This study reports the identification of 10 microRNA (miRNA) genes, Bhv1-mir-B1–Bhv1-mir-B10, encoded by the BoHV-1 genome that were processed into 12 detectable mature miRNAs as determined by ultra-high throughput sequencing bioinformatics analyses of small RNA libraries and expression studies. We found that four of the miRNA genes were present as two copies in the BoHV-1 genome, resulting in a total of 14 miRNA encoding loci. Unique features of the BoHV-1 miRNAs include evidence of bidirectional transcription and a close association of two miRNA genes with the origin of replication, including one miRNA that is encoded within the origin of replication. The miRNA gene Bhv1-mir-B5 was encoded on the opposite DNA strand to the latency associated transcript, potentially giving rise to antisense transcripts originating from this locus. The association of herpesvirus miRNAs with latency appears to be a common feature in the alphaherpesviruses. Analyses of the BoHV-5 genome for putative miRNA gene orthologues identified a high degree of evolutionary conservation for nine of the BoHV-1 miRNA genes. The possible roles for BoHV-1 miRNAs in the regulation of known BoHV-1 transcription units and the genetics of the BoHV-1 genotypes are also discussed.

Item ID:	47636
Item Type:	Article (Research - C1)
ISSN:	1465-2099
Funders:	Queensland Primary Industries and Fisheries (QPIF), University of Queensland (UQ), Australian Government (AG)
Projects and Grants:	UQ Early Career Research Grant, AG Endeavour Research Fellowship
Date Deposited:	08 Mar 2017 21:35
FoR Codes:	11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 100%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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