K+ currents activated by depolarization in cardiac fibroblasts

Shibukawa, Yoshiyuki, Chilton, E. Lisa, MacCannell, K. Andrew, Clark, Robert B., and Giles, Wayne R. (2005) K+ currents activated by depolarization in cardiac fibroblasts. Biophysical Journal, 88 (6). pp. 3924-3935.

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K+ currents expressed in freshly dispersed rat ventricular fibroblasts have been studied using whole-cell patch-clamp recordings. Depolarizing voltage steps from a holding potential of −90 mV activated time- and voltage-dependent outward currents at membrane potentials positive to not, vert, similar−30 mV. The relatively slow activation kinetics exhibited strong dependence on the membrane potential. Selected changes in extracellular K+ concentration ([K+]o) revealed that the reversal potentials of the tail currents changed as expected for a K+ equilibrium potential. The activation and inactivation kinetics of this K+ current, as well as its recovery from inactivation, were well-fitted by single exponential functions. The steady-state inactivation was well described by a Boltzmann function with a half-maximal inactivation potential (V0.5) of −24 mV. Increasing [K+]o (from 5 to 100 mM) shifted this V0.5 in the hyperpolarizing direction by −11 mV. Inactivation was slowed by increasing [K+]o to 100 mM, and the rate of recovery from inactivation was decreased after increasing [K+]o. Block of this K+ current by extracellular tetraethylammonium also slowed inactivation. These [K+]o-induced changes and tetraethylammonium effects suggest an important role for a C-type inactivation mechanism. This K+ current was sensitive to dendrotoxin-I (100 nM) and rTityustoxin Kα (50 nM).

Item ID: 4708
Item Type: Article (Research - C1)
ISSN: 1542-0086
Date Deposited: 07 Sep 2009 03:42
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1116 Medical Physiology > 111603 Systems Physiology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 100%
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