Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification

Soli, Kevin W., Kas, Monalisa, Maure, Tobias, Umezaki, Masahiro, Morita, Ayako, Siba, Peter M., Greenhill, Andrew R., and Horwood, Paul F. (2013) Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification. Diagnostic Microbiology and Infectious Disease, 77 (4). pp. 321-323.

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Abstract

We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.

Item ID: 46993
Item Type: Article (Research - C1)
ISSN: 1879-0070
Keywords: LAMP; loop-mediated isothermal amplification; colorimetric detection; hydroxynaphthol blue; SYBR Green; Salmonella; Shigella; Vibrio cholerae
Date Deposited: 08 Feb 2017 22:23
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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