Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8⁺ T-cell responses

Apte, Simon H., Stephenson, Rachel J., Simerska, Pavla, Groves, Penny L., Aljohani, Salwa, Eskandari, Sharareh, Toth, Istvan, and Doolan, Denise L. (2016) Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8⁺ T-cell responses. Nanomedicine, 11 (2). pp. 137-152.

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Abstract

Aim: Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8⁺ T-cell responses.

Materials & methods: Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8⁺ T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8⁺ T-cell responses and inhibit tumor growth in vivo.

Results: The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation.

Conclusion: The C12 polylysine platform was an effective configuration for induction of potent CD8⁺ T-cell responses.

Item ID: 46649
Item Type: Article (Refereed Research - C1)
Keywords: CD8+ T cells; dendritic cells; lipid core peptide; nanovaccine; self-adjuvant; vaccine
ISSN: 1748-6963
Funders: National Health and Medical Research Council (NHMRC), Australian Research Council (ARC), University of Queensland (UQ)
Projects and Grants: NHMRC APP1037304, NHMRC APP1026488, NHMRC 1023636, ARC Discovery Project Grant DP1092829, ARC DP110100212, UQ 606431
Date Deposited: 15 Dec 2016 02:45
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111716 Preventive Medicine @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 25%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110705 Humoural Immunology and Immunochemistry @ 25%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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