Apte, Simon H., Stephenson, Rachel J., Simerska, Pavla, Groves, Penny L., Aljohani, Salwa, Eskandari, Sharareh, Toth, Istvan, and Doolan, Denise L. (2016) Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8⁺ T-cell responses. Nanomedicine, 11 (2). pp. 137-152.

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Abstract

Aim: Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8⁺ T-cell responses.

Materials & methods: Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8⁺ T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8⁺ T-cell responses and inhibit tumor growth in vivo.

Results: The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation.

Conclusion: The C12 polylysine platform was an effective configuration for induction of potent CD8⁺ T-cell responses.

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