Identification of bioactives from the red seaweed Asparagopsis taxiformis that promote antimethanogenic activity in vitro

Machado, Lorenna, Magnusson, Marie, Paul, Nicholas A., Kinley, Robert, de Nys, Rocky, and Tomkins, Nigel (2016) Identification of bioactives from the red seaweed Asparagopsis taxiformis that promote antimethanogenic activity in vitro. Journal of Applied Phycology, 28 (5). pp. 3117-3126.

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Abstract

Asparagopsis taxiformis has potent antimethanogenic activity as a feed supplement at 2 % of organic matter in in vitro bioassays. This study identified the main bioactive natural products and their effects on fermentation using rumen fluid from Bos indicus steers. Polar through to non-polar extracts (water, methanol, dichloromethane and hexane) were tested. The dichloromethane extract was most active, reducing methane production by 79 %. Bromoform was the most abundant natural product in the biomass of Asparagopsis (1723 μg g⁻¹ dry weight [DW] biomass), followed by dibromochloromethane (15.8 μg g⁻¹ DW), bromochloroacetic acid (9.8 μg g⁻¹ DW) and dibromoacetic acid (0.9 μg g⁻¹ DW). Bromoform and dibromochloromethane had the highest activity with concentrations ≥1 μM inhibiting methane production. However, only bromoform was present in sufficient quantities in the biomass at 2 % organic matter to elicit this effect. Importantly, the degradability of organic matter and volatile fatty acids were not affected at effective concentrations.

Item ID: 46382
Item Type: Article (Research - C1)
ISSN: 1573-5176
Keywords: red macroalgae, rhodophyta, seaweed, natural products, methane inhibitors, bromoform
Funders: Australian Government Department of Agriculture (AG-DA), Australian Renewable Energy Agency (ARENA), Advanced Manufacturing Cooperative Research Centre (AMCRC)
Date Deposited: 09 Nov 2016 07:36
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070204 Animal Nutrition @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8398 Environmentally Sustainable Animal Production > 839802 Management of Greenhouse Gas Emissions from Animal Production @ 100%
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