The Anisakis Transcriptome provides a resource for fundamental and applied studies on allergy-causing parasites

Baird, Fiona J., Su, Xiaopei, Aibinu, Ibukun, Nolan, Matthew J., Sugiyama, Hiromu, Otranto, Domenico, Lopata, Andreas L., and Cantacessi, Cinzia (2016) The Anisakis Transcriptome provides a resource for fundamental and applied studies on allergy-causing parasites. PLoS Neglected Tropical Diseases, 10 (7). e0004845. pp. 1-17.

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Abstract

Background: Food-borne nematodes of the genus Anisakis are responsible for a wide range of illnesses (=anisakiasis), from self-limiting gastrointestinal forms to severe systemic allergic reactions, which are often misdiagnosed and under-reported. In order to enhance and refine current diagnostic tools for anisakiasis, knowledge of the whole spectrum of parasite molecules transcribed and expressed by this parasite, including those acting as potential allergens, is necessary.

Methodology/Principal Findings: In this study, we employ high-throughput (Illumina) sequencing and bioinformatics to characterise the transcriptomes of two Anisakis species, A. simplex and A. pegreffii, and utilize this resource to compile lists of potential allergens from these parasites. A total of similar to 65,000,000 reads were generated from cDNA libraries for each species, and assembled into similar to 34,000 transcripts (=Unigenes); similar to 18,000 peptides were predicted from each cDNA library and classified based on homology searches, protein motifs and gene ontology and biological pathway mapping. Using comparative analyses with sequence data available in public databases, 36 (A. simplex) and 29 (A. pegreffii) putative allergens were identified, including sequences encoding `novel' Anisakis allergenic proteins (i.e. cyclophilins and ABA-1 domain containing proteins).

Conclusions/Significance: This study represents a first step towards providing the research community with a curated dataset to use as a molecular resource for future investigations of the biology of Anisakis, including molecules putatively acting as allergens, using functional genomics, proteomics and immunological tools. Ultimately, an improved knowledge of the biological functions of these molecules in the parasite, as well as of their immunogenic properties, will assist the development of comprehensive, reliable and robust diagnostic tools.

Item ID: 45780
Item Type: Article (Research - C1)
ISSN: 1935-2735
Additional Information:

© 2016 Baird et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: James Cook University (JCU), Australian Research Council (ARC), National Health and Medical Research Council of Australia (NHMRC), Isaac Newton Trust/Wellcome Trust ISSF/University of Cambridge Joint Research Grants Scheme (JRGS), Royal Society (RS)
Projects and Grants: JRGS PNVM/GAAB, RS PNAG/428
Date Deposited: 14 Sep 2016 07:34
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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