A next-generation proteome array for Schistosoma mansoni

de Assis, Rafael Ramiro, Ludolf, Fernanda, Nakajima, Rie, Jasinskas, Al, Oliveira, Guilherme C., Felgner, Philip, Gaze Jangola, Soraya T., Loukas, Alex, LoVerde, Philip T., Bethony, Jeffrey M., Correa-Oliveira, Rodrigo, and Calzavara-Silva, Carlos E. (2016) A next-generation proteome array for Schistosoma mansoni. International Journal for Parasitology, 46 (7). pp. 411-415.

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Abstract

A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

Item ID: 45544
Item Type: Article (Research - C1)
ISSN: 0020-7519
Keywords: Schistosoma mansoni, protein microarray, proteome, antibody signature
Funders: National Institutes of Health (NIH), National Council for Scientific and Technological Development, Brazil (CNPq)
Projects and Grants: NIH grant number U01 R01-A1095916-04, NIH grant number U19-A1089686-05, NIH grant number P50A1098507, CNPq 478131/2012-7, CNPq 168260/2014-0
Date Deposited: 03 Aug 2016 07:39
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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