Characterization of the hemoglobin and red blood cell system in the copper rockfish, Sebastes caurinus

Rummer, J.L., Regan, M., and Brauner, C.J. (2006) Characterization of the hemoglobin and red blood cell system in the copper rockfish, Sebastes caurinus. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 143 (Suppl 4). S74-S74.

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Abstract

The North American Pacific coast harbors almost 100 rockfish species, some of which are the most long-lived teleosts (and vertebrates) on record! The combination of being long-lived, slowgrowing, deep-dwelling, and genetically isolated makes rockfish a prime candidate for conservation and fascinating for physiological research. We investigated hemoglobin (Hb) oxygen (O₂) binding properties, the Root effect, and the b-adrenergic red blood cell (RBC) response in copper rockfish, Sebastes caurinus (260–680 g wet weight). In vitro, copper rockfish RBCs possess a pronounced Bohr effect (B=_1.2) resulting in a substantial right shift in the O₂ equilibrium curve (P50 from approximately 24 to 123 mm Hg with an increase in PCO₂ from 3.8 to 15.2 mm Hg, respectively). The Root effect is substantial, and onset is initiated near resting in vivo pH values. Our data are in agreement with the only other published study to date where the Root effect was characterized with respect to depth distribution and swimming activity in seven different Sebastes species. Finally, as expected, the RBC stress response is b-adrenergic and results in significant RBC swelling, elevation of intracellular pH, and reduction in extracellular pH. Our data provide a foundation for understanding respiratory physiology of an interesting deep-water, advanced teleost. Unfortunately, rockfish are severely over-fished and highly prized in Asian markets. Currently, copper rockfish are being investigated as a candidate species in aquaculture, which will not only help meet commercial demand for this species while simultaneously reducing fishing pressure in wild stocks, it will provide easy access to an intriguing group of fish for further scientific investigation.

Item ID: 45316
Item Type: Article (Abstract)
ISSN: 1531-4332
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Abstract from the Annual Main Meeting of the Society for Experimental Biology, University of Kent at Canterbury, UK, 2nd-7th April, 2006

Date Deposited: 05 Sep 2016 23:08
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960802 Coastal and Estuarine Flora, Fauna and Biodiversity @ 100%
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