Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus

Rush, C., Hafner, L., and Timms, P. (1997) Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus. Applied Microbiology and Biotechnology, 47 (5). pp. 537-542.

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An expression system based on the Staphylococcus aureus protein A gene (spa) was developed to allow the production and export of proteins in Lactobacillus. Plasmid shuttle vectors were constructed that carried the eZZ gene, a synthetic gene based on the Protein A gene (spa) but lacking the carboxy-terminal membrane-anchoring region. A gene fusion was created between the eZZ gene and the VD4 region of a chlamydial major outer-membrane protein gene. Expression studies demonstrated the recognition of the spa regulatory signals by several Lactobacillus, with the recombinant protein being expressed (from 0.1 mu g of EZZVD4 fusion protein per mi in L. plantarum up to 10 mu g of EZZ protein per mi in L. fermentum) and exported (levels up to 20% in L. fermentum) in several Lactobacillus strains.

Item ID: 44979
Item Type: Article (Research - C1)
ISSN: 1432-0614
Keywords: Lactobacillus; vaccine; recombinant antigen, vector
Funders: National Health and Medical Research Council of Australia (NHMRC), Queensland University of Technology
Date Deposited: 03 Aug 2016 03:53
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110702 Applied Immunology (incl Antibody Engineering, Xenotransplantation and T-cell Therapies) @ 10%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 50%
10 TECHNOLOGY > 1004 Medical Biotechnology > 100499 Medical Biotechnology not elsewhere classified @ 40%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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