Ricci, Susanna, Medaglini, Donata, Rush, Catherine M., Marcello, Alessandro, Peppoloni, Samuele, Manganelli, Riccardo, Palú, Giorgio, and Pozzi, Gianni (2000) Immunogenicity of the B monomer of Escherichia coli heat-labile toxin expressed on the surface of Streptococcus gordonii. Infection and Immunity, 68 (2). pp. 760-766.

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Abstract

The B monomer of the Escherichia coli heat-labile toxin (LTB) was expressed on the surface of the human oral commensal bacterium Streptococcus gordonii. Recombinant bacteria expressing LTB were used to immunize BALB/c mice subcutaneously and intragastrically. The LTB monomer expressed on the streptococcal surface proved to be highly immunogenic, as LTB-specific immunoglobulin G (Igc) serum titers of 140,000 were induced after systemic immunization. Most significantly, these antibodies were capable of neutralizing the enterotoxin in a cell neutralization assay. Following mucosal delivery, antigen-specific IgA antibodies were found in feces and antigen-specific IgG antibodies were found in sera. Analysis of serum Ige subclasses showed a clear predominance of IgG1 when recombinant bacteria were inoculated subcutaneously, while a prevalence of IgG2a was observed upon intragastric delivery, suggesting, in this case, the recruitment of a Th1 type of immune response.

Item ID:	44975
Item Type:	Article (Research - C1)
ISSN:	1098-5522
Keywords:
Funders:	Commission of the European Union (CEU), Istituto Superiore di Sanità (ISS), CNR
Projects and Grants:	CEU BIOTECH contract BIO2 CT94-3055, CEU BIOTECH contract BIO3 CT96-0542, ISS Progetto AIDS contract no. 40A.0.83, CNR P.F. Biotecnologie, contract no. 97.01185.PF49
Date Deposited:	03 Aug 2016 02:21
FoR Codes:	11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 50% 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 50%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50% 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 50%
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