Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using illumina sequencing

Trapnell, Dorset W., Beasley, Rochelle R., Lance, Stacey L., Field, Ashley, and Jones, Kenneth L. (2015) Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using illumina sequencing. Applications in Plant Sciences, 3 (6). pp. 1-4.

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Abstract

Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.

Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ~80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.

Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.

Item ID: 43379
Item Type: Article (Refereed Research - C1)
Keywords: Dendrobium calamiforme; Dockrillia calamiformis; genetic diversity; Orchidaceae; phylogeography; simple sequence repeat (SSR) marker
Additional Information:

© 2015 Trapnell et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).

ISSN: 2168-0450
Funders: University of Georgia, US Department of Energy (DOE)
Projects and Grants: DEO DEFC09-07SR22506
Research Data: http://www.bioone.org/doi/full/10.3732/apps.1500016
Date Deposited: 19 Jun 2016 05:10
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 40%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy @ 60%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960803 Documentation of Undescribed Flora and Fauna @ 100%
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