Proteins of bovine ephemeral fever virus

Walker, Peter J., Byrne, Keren A., Cybinski, D. H., Doolan, Denise, and Wang, Yonghong (1991) Proteins of bovine ephemeral fever virus. Journal of General Virology, 72. pp. 67-74.

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Abstract

The proteins of bovine ephemeral fever virus (BEFV) were examined in purified virions and in infected BHK-21 cells. Five structural proteins were named L (180K), G (81K), N (52K), M₁ (43K) and M₂ (29K). The 81 K G protein incorporated [³H]glucosamine, was removed from virions by treatment with Triton X-100 and bound monoclonal antibodies which were both neutralizing and protective. Treatment of virions with Triton X-100 and 0.2 to 1.0 m-NaCl progressively released L, M₁ and M₂. The N protein remained associated with nucleocapsids in up to 2.5 m-NaCl. The glycoprotein (G), nucleoprotein (N) and matrix protein (M₂) were phosphorylated. In BEFV-infected BHK-21 cells, five virus-induced proteins were detected from 12 h post-infection. The L, N, M₁ and M₂ proteins corresponded to those detected in virions whereas the G protein existed in two forms. In tunicamycin-treated cells these occurred as 67K and 71K non-glycosylated precursors. In the absence of tunicamycin, 77K and 79K glycosylated forms were further modified to produce the 81K virion G protein and a 90K cell-associated form. Five viral proteins were also detected in cells infected with the closely related Berrimah virus; the Berrimah virus G protein was also present in two forms.

Item ID: 42787
Item Type: Article (Refereed Research - C1)
ISSN: 1465-2099
Funders: Australian Centre for International Agricultural Research (ACIAR)
Date Deposited: 23 Mar 2016 01:27
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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