Location of human cytotoxic T cell epitopes within a polymorphic domain of the Plasmodium falciparum circumsporozoite protein

Doolan, Denise L., Houghten, Richard A., and Good, Michael F. (1991) Location of human cytotoxic T cell epitopes within a polymorphic domain of the Plasmodium falciparum circumsporozoite protein. International Immunology, 3 (6). pp. 511-516.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1093/intimm/3.6.511
 
3


Abstract

Studies in mice have shown that cytotoxic T lymphocytes (CTL) specific for epitopes within the circumsporozoite (CS) protein of malaria sporozoltes can prevent malaria probably by destroying infected hepatocytes. This has provided a model for the development of a sporozoite vaccine. It has not been shown whether humans can mount a CTL response to this protein nor what determinants on the protein could be considered as target epitopes for such cells and thus merit Inclusion in a sporozoite vaccine. We have used a novel technique to study a Caucasian population which would benefit from a sporozoite vaccine and have been able to demonstrate that some Individuals with a history of sporozoite exposure do contain peripheral blood CTL specific for the Plasmodium falciparum CS protein. The prevalence of CTL among different Individuals Is low and there is evidence that recent malaria exposure may be a prerequisite for finding such CTL. In three Individuals, CTL could be repeatedly found and in all cases the epttopes mapped to one of the two polymorphic C-terminal domains. Using a CTL line, we mapped a recognition site to residues 351 – 395 of the CS protein, overlapping the region of the protein recognized by murine CTL.

Item ID: 42786
Item Type: Article (Refereed Research - C1)
Keywords: malana vaccines, sporozoite immunity
ISSN: 1460-2377
Funders: National Health and Medical Research Council (NHMRC)
Date Deposited: 23 Mar 2016 01:14
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
Downloads: Total: 3
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page