Induction of CD4⁺ T cell-dependent CD8⁺ type 1 responses in humans by a malaria DNA vaccine

Wang, Ruobing, Epstein, Judith, Baraceros, Fe Maria, Gorak, Edward J., Charoenvit, Yupin, Carucci, Daniel J., Hedstrom, Richard C., Rahardjo, Nancy, Gay, Tanya, Hobart, Peter, Stout, Rick, Jones, Trevor R., Richie, Thomas L., Parker, Suezanne E., Doolan, Denise L., Norman, Jon, and Hoffman, Stephen L. (2001) Induction of CD4⁺ T cell-dependent CD8⁺ type 1 responses in humans by a malaria DNA vaccine. Proceedings of the National Academy of Sciences of the United States of America, 98 (19). pp. 10817-10822.

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We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-γ responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8⁺ and CD4⁺ T cells. Overall, frequency of response was significantly greater after i.m. jet injection. CD8⁺-dependent cytotoxic T lymphocytes (CTL) were detected in 8/14 volunteers. Demonstration in humans of elicitation of the class I restricted IFN-γ responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine.

Item ID: 42766
Item Type: Article (Research - C1)
ISSN: 1091-6490
Funders: Naval Medical Research and Development Command (NMRDC), United States Agency for International Development (USAID), Office of Naval Research Advanced Technology Demonstration (ONRATD)
Projects and Grants: NMRDC 61102A.S13.F.A0009, NMRDC 62787A.870.F.A0010, NMRDC 63002A.810.F.A0011, NMRDC 603792N.01889.135.A0039 , NMRDC 60000.000.000.A0062
Date Deposited: 23 Mar 2016 05:09
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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