Utilization of genomic sequence information to develop malaria vaccines

Doolan, D.L., Aguiar, J.C., Weiss, W.R., Sette, A., Felgner, P.L., Regis, D.P., Quinones-Casas, P., Yates, J.R., Blair, P.L., Richie, T.L., Hoffman, S.L., and Carucci, D.J. (2003) Utilization of genomic sequence information to develop malaria vaccines. Journal of Experimental Biology, 206. pp. 3789-3802.

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Recent advances in the fields of genomics, proteomics and molecular immunology offer tremendous opportunities for the development of novel interventions against public health threats, including malaria. However, there is currently no algorithm that can effectively identify the targets of protective T cell or antibody responses from genomic data. Furthermore, the identification of antigens that will stimulate the most effective immunity against the target pathogen is problematic, particularly if the genome is large. Malaria is an attractive model for the development and validation of approaches to translate genomic information to vaccine development because of the critical need for effective anti-malarial interventions and because the Plasmodium parasite is a complex multistage pathogen targeted by multiple immune responses. Sterile protective immunity can be achieved by immunization with radiation-attenuated sporozoites, and anti-disease immunity can be induced in residents in malaria-endemic areas. However, the 23 Mb Plasmodium falciparum genome encodes more than 5300 proteins, each of which is a potential target of protective immune responses. The current generation of subunit vaccines is based on a single or few antigens and therefore might elicit too narrow a breadth of response. We are working towards the development of a new generation vaccine based on the presumption that duplicating the protection induced by the whole organism may require a vaccine nearly as complex as the organism itself. Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development.

Item ID: 42756
Item Type: Article (Research - C1)
ISSN: 1477-9145
Keywords: Plasmodium, P. falciparum, vaccine, genomics, proteomics, molecular immunology, immune screening, multiepitope
Funders: Naval Medical Research and Development Command (NMRDC)
Projects and Grants: NMRDC Work Unit No. 61102A.S13.F.A0009, NMRDC Work Unit No. 62787A.870.F.A0228
Date Deposited: 15 Sep 2016 00:46
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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