Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data

Doolan, Denise L., Southwood, Scott, Freilich, Daniel A., Sidney, John, Graber, Norma L., Shatney, Lori, Bebris, Lolita, Florens, Laurence, Dobano, Carlota, Witney, Adam A., Appella, Ettore, Hoffman, Stephen L., Yates, John R., Carucci, Daniel J., and Sette, Alessandro (2003) Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data. Proceedings of the National Academy of Sciences of the United States of America, 100 (17). pp. 9952-9957.

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Abstract

The recent explosion in genomic sequencing has made available a wealth of data that can now be analyzed to identify protein antigens, potential targets for vaccine development. Here we present, in the context of Plasmodium falciparum, a strategy that rapidly identifies target antigens from large and complex genomes. Sixteen antigenic proteins recognized by volunteers immunized with radiation-attenuated P. falciparum sporozoites, but not by mock immunized controls, were identified. Several of these were more antigenic than previously identified and well characterized P. falciparum-derived protein antigens. The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens.

Item ID: 42754
Item Type: Article (Refereed Research - C1)
ISSN: 1091-6490
Date Deposited: 15 Sep 2016 00:25
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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