Vaxfectin™ enhances immunogenicity and protective efficacy of P. yoelii circumsporozoite DNA vaccines

Sedegah, Martha, Rogers, William O., Belmonte, Arnel, Belmonte, Maria, Banania, Glenna, Patterson, Noelle, Ferrari, Marilyn, Kaslow, David C., Carucci, Daniel J., Richie, Thomas L., and Doolan, Denise L. (2006) Vaxfectin™ enhances immunogenicity and protective efficacy of P. yoelii circumsporozoite DNA vaccines. Vaccine, 24 (11). pp. 1921-1927.

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Abstract

We evaluated the capacity of the cationic lipid based formulation, Vaxfectin™, to enhance the immunogenicity and protective efficacy of DNA-based vaccine regimens in the Plasmodium yoelii murine malaria model. We immunized Balb/c mice with varying doses (0.4–50 μg) of plasmid DNA (pDNA) encoding the P. yoelii circumsporozoite protein (PyCSP), either in a homologous DNA/DNA regimen (D-D) or a heterologous prime-boost DNA-poxvirus regimen (D-V). At the lowest pDNA doses, Vaxfectin™ substantially enhanced IFA titers, ELISPOT frequencies, and protective efficacy. Clinical trials of pDNA vaccines have often used low pDNA doses based on a per kilogram weight basis. Formulation of pDNA vaccines in Vaxfectin™ may improve their potency in human clinical trials.

Item ID: 42751
Item Type: Article (Refereed Research - C1)
Keywords: malaria; T-lymphocytes; plasmid DNA (pDNA) vaccines
ISSN: 1873-2518
Funders: Naval Medical Research and Development Command (NMRDC), CRADA with Vical Inc. (CRADA-VI)
Projects and Grants: NMRDC 61102A3M161102.BS13 AK111, NMRDC 62787A 3M162787.A870 AN121, (CRADA-VI) Agreement Number: NCRADA-NMRC-03-1561
Date Deposited: 21 Mar 2016 22:51
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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