Dobaño, Carlota, Widera, Georg, Rabussay, Dietmar, and Doolan, Denise L. (2007) Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation. Vaccine, 25 (36). pp. 6635-6645.

PDF (Published Version) - Published Version Restricted to Repository staff only

Abstract

We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 μg of DNA via EP was equivalent to 50 μg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-γ responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.

Item ID:	42739
Item Type:	Article (Research - C1)
ISSN:	1873-2518
Keywords:	malaria; DNA vaccine; electroporation
Funders:	Naval Medical Research Centre (NMRC), Office of Naval Research (ONR)
Projects and Grants:	ONR 62787A.870.F. A0224, ONR 62787A,977.F. A0238
Date Deposited:	21 Mar 2016 23:52
FoR Codes:	11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
Downloads:	Total: 3
	More Statistics