Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation

Dobaño, Carlota, Widera, Georg, Rabussay, Dietmar, and Doolan, Denise L. (2007) Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation. Vaccine, 25 (36). pp. 6635-6645.

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Abstract

We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 μg of DNA via EP was equivalent to 50 μg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-γ responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.

Item ID: 42739
Item Type: Article (Refereed Research - C1)
Keywords: malaria; DNA vaccine; electroporation
ISSN: 1873-2518
Funders: Naval Medical Research Centre (NMRC), Office of Naval Research (ONR)
Projects and Grants: ONR 62787A.870.F. A0224, ONR 62787A,977.F. A0238
Date Deposited: 21 Mar 2016 23:52
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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