Dinuclear ruthenium(II) complexes containing one inert metal centre and one coordinatively-labile metal centre: syntheses and biological activities

Li, Xin, Heimann, Kirsten, Li, Fangfei, Warner, Jeffrey M., Keene, F. Richard, and Collins, J. Grant (2016) Dinuclear ruthenium(II) complexes containing one inert metal centre and one coordinatively-labile metal centre: syntheses and biological activities. Dalton Transactions, 45. pp. 4017-4029.

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Abstract

A series of non-symmetric dinuclear polypyridylruthenium(II) complexes (Rubbₙ-Cl) that contain one inert metal centre and one coordinatively-labile metal centre, linked by the bis[4(4'-methyl-2,2'-bipyridyl)]-1,n-alkane ligand ("bbₙ" for n = 7, 12 and 16), have been synthesised and their potential as antimicrobial agents examined. The minimum inhibitory concentrations (MIC) of the ruthenium(II) complexes were determined against four strains of bacteria − Gram-positive Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA), and Gram-negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). The Rubbₙ-Cl complexes displayed good antimicrobial activity, with Rubb₁₂-Cl being the most active complex against both Gram-positive and Gram-negative strains. Interestingly, Rubb₇-Cl was found to be eight- and sixteen-fold more active towards E. coli than against S. aureus and MRSA, respectively. The cytotoxicities of the Rubbₙ-Cl complexes against three eukaryotic cell lines − two kidney cell lines (BHK and HEK-293) and one liver cell line (HepG2) − were examined. The Rubbₙ-Cl complexes were found to be considerably less toxic towards eukaryotic cells than S. aureus, MRSA and E. coli, with Rubb₁₂-Cl being thirty- to eighty-times more toxic to the bacteria than to BHK, HEK-293 or HepG2 cells. Unexpectedly, Rubb₇-Cl was far more toxic to HepG2 cells (24h-IC₅₀ = 3.7 μM) and far less toxic to BHK cells (24h-IC₅₀ = 238 μM) than the Rubb₁₂-Cl and Rubb₁₆-Cl complexes. In order to understand the unexpected large differences in the cytotoxicities of the Rubbₙ-Cl complexes towards eukaryotic cells, a confocal microscopic study of their intracellular localisation was undertaken. The results suggest that the observed cytotoxicity might be related to the extent of DNA binding.

Item ID: 42399
Item Type: Article (Refereed Research - C1)
Keywords: antibacterial; polypyridylruthenium; cytotoxity; eukaryotic cells; confocal microscopy; cellular localisation
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ISSN: 1477-9234
Funders: University of New South Wales, Canberra
Date Deposited: 09 Feb 2016 04:49
FoR Codes: 03 CHEMICAL SCIENCES > 0302 Inorganic Chemistry > 030201 Bioinorganic Chemistry @ 70%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 30%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 70%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 30%
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