Cytological defects during embryogenesis in heat-induced tetraploid Kuruma shrimp Penaeus japonicus

Foote, Andrew, Sellars, Melony, Coman, Greg, and Merritt, David (2010) Cytological defects during embryogenesis in heat-induced tetraploid Kuruma shrimp Penaeus japonicus. Arthropod Structure & Development, 39 (4). pp. 268-275.

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Tetraploid shrimp embryos have been induced: however, in all cases no postlarvae were produced. This study determined when tetraploid Penaeus japonicus became non-viable and identified unique abnormalities to aid in elucidating the causes of lethality. Embryonic development was analyzed using flow cytometry to determine ploidy and laser scanning confocal microscopy for cytological examination of embryogenesis. Abnormalities exclusive to tetraploids were identified from the 1-cell stage: an off-centre pronucleus, polypolar spindles, delayed time to first mitosis and polypolar cleavage. Following first mitosis in the tetraploids, 50% of the cells did not contain DNA. This unique abnormality was not resolved later in development and is therefore believed to be a lethal trait. Causes of this phenomenon likely stemmed from abnormal mitotic spindle regeneration following the mitotic heat shock. Consequently, the findings of this study indicate that current methods of tetraploidy induction using heat shock appear unsuitable for viable tetraploid shrimp production.

Item ID: 42125
Item Type: Article (Research - C1)
ISSN: 1873-5495
Keywords: tetraploid; shrimp; intracellular body; anucleate; tetraploar spindles
Date Deposited: 16 Dec 2015 04:21
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060403 Developmental Genetics (incl Sex Determination) @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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