Intermittent preventive treatment with sulfadoxine-pyrimethamine does not modify plasma cytokines and chemokines or intracellular cytokine responses to Plasmodium falciparum in Mozambican Children

Quelhas, Diana, Puyol, Laura, Quintó, Llorenç, Nhampossa, Tacilta, Serra-Casas, Elisa, Macete, Eusébio, Aide, Pedro, Sanz, Sergi, Aponte, John J., Doolan, Denise L., Alonso, Pedro L., Menéndez, Clara, and Dobaño, Carlota (2012) Intermittent preventive treatment with sulfadoxine-pyrimethamine does not modify plasma cytokines and chemokines or intracellular cytokine responses to Plasmodium falciparum in Mozambican Children. BMC Immunology, 13 (5). pp. 1-18.

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Abstract

Background: Cytokines and chemokines are key mediators of anti-malarial immunity. We evaluated whether Intermittent Preventive Treatment in infants with Sulfadoxine-Pyrimethamine (IPTi-SP) had an effect on the acquisition of these cellular immune responses in Mozambican children. Multiple cytokines and chemokines were quantified in plasma by luminex, and antigen-specific cytokine production in whole blood was determined by intracellular cytokine staining and flow cytometry, at ages 5, 9, 12 and 24 months.

Results: IPTi-SP did not significantly affect the proportion of CD3+ cells producing IFN-γ, IL-4 or IL-10. Overall, plasma cytokine or chemokine concentrations did not differ between treatment groups. Th1 and pro-inflammatory responses were higher than Th2 and anti-inflammatory responses, respectively, and IFN-γ:IL-4 ratios were higher for placebo than for SP recipients. Levels of cytokines and chemokines varied according to age, declining from 5 to 9 months. Plasma concentrations of IL-10, IL-12 and IL-13 were associated with current infection or prior malaria episodes. Higher frequencies of IFN-γ and IL-10 producing CD3+ cells and elevated IL-10, IFN-γ, MCP-1 and IL-13 in plasma were individually associated with increased malaria incidence, at different time points. When all markers were analyzed together, only higher IL-17 at 12 months was associated with lower incidence of malaria up to 24 months.

Conclusions: Our work has confirmed that IPTi-SP does not negatively affect the development of cellular immune response during early childhood. This study has also provided new insights as to how these cytokine responses are acquired upon age and exposure to P. falciparum, as well as their associations with malaria susceptibility.

Item ID: 41437
Item Type: Article (Refereed Research - C1)
Keywords: cytokines; chemokines; IPTi; falciparum malaria; sulfadoxine-pyrimethamine
Additional Information:

© Quelhas et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

ISSN: 1471-2172
Funders: Bill and Melinda Gates Foundation (BMGF), Banco de Bilbao-Vizcaya-Argentaria Foundation (BBVAF), World Health Organisation (WHO), Ministerio de Ciencia e Innovación (MCI), Pfizer Australia Senior Research Fellowship (PASRF)
Projects and Grants: BMGF IPTi03-0, BBVAF BBVA 02-0, WHO OD/TS-07-00017, MCI RYC-2008-02631
Date Deposited: 24 Mar 2016 04:46
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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