The efficacy of extraembryonic stem cells in improving blood flow within animal models of lower limb ischaemia

Mohamed Omer, Safraz, Krishna, Smriti Murali, Li, Jiaze, Moxon, Joseph Vaughan, Nsengiyumva, Vianne, and Golledge, Jonathan (2016) The efficacy of extraembryonic stem cells in improving blood flow within animal models of lower limb ischaemia. Heart, 102 (1). pp. 69-74.

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Background: Stem cell (SC) administration is a potential therapeutic strategy to improve blood supply in patients with peripheral artery disease (PAD). The aim of this systematic review and meta-analysis was to investigate the efficacy of extraembryonic tissue-derived SC (ETSC) in improving blood flow within animal models of hindlimb ischaemia (HLI).

Methods: PubMed, ScienceDirect and Web of Science were searched to identify studies which investigated ETSCs within animal HLI models. A meta-analysis was performed focusing on the effect of ETSCs on limb blood flow assessed by laser Doppler imaging using a random effects model. Methodological quality was assessed using a newly devised quality assessment tool.

Results: Five studies investigating umbilical cord-derived SCs (three studies), placental SCs (one study), amnion and chorionic SCs (one study) were included. A meta-analysis suggested that administration of ETSCs improved the restoration of blood flow within the HLI models used. The methodological quality of the included studies was assessed as poor. Problems identified included lack of randomised design and blinding of outcome assessors; that the animal models did not incorporate recognised risk factors for human PAD or atherosclerosis; the models used did not have established chronic ischaemia as is the cases in most patients presenting with PAD; and the studies lacked a clear rationale for the dosage and frequency of SCs administered.

Conclusions: The identified studies suggest that ETSCs improve recovery of limb blood supply within current animal HLI models. Improved study quality is, however, needed to provide support for the likelihood of translating these findings to patients with PAD.

Item ID: 41349
Item Type: Article (Research - C1)
ISSN: 1468-201X
Funders: National Health and Medical Research Council (NHMRC), Queensland Government, Townsville Hospital Private Practice Trust Fund (THPPTF), James Cook University (JCU), National Centre for Research Excellence in Peripheral Arterial Disease, Queensland Research Centre for Peripheral Vascular Diseases
Projects and Grants: NHMRC 1079369, NHMRC 1079193, NHMRC 1063476, NHMRC 1021416, NHMRC 1000967, NHMRC 1019921
Date Deposited: 15 Mar 2016 22:31
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110399 Clinical Sciences not elsewhere classified @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiovascular Medicine and Haematology > 110299 Cardiovascular Medicine and Haematology not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 50%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 50%
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